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Berkala Penelitian Hayati
Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 8 Documents
 1 
Search results for , issue "Vol 28 No 1 (2022): December 2022" : 8 Documents clear
Infestation and host specificity of mistletoe parasitic plants in Purwodadi Botanic Garden Solikin Solikin
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 28 No 1 (2022): December 2022
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.28.1.20221

Abstract

Mistletoes are aerial hemiparasitic plant that infesting and parasitizing on wild and cultivated plants. They are can be categorized as generalist and specialist mistletoes according to their host plants diversity. Shannon index of the host plants diversity can be used to determine host specificity of mistletoes. Study aimed to know and determine host plants and host specificity of mistletoes in Purwodadi Botanic Garden was conducted in 2013, 2017 and 2021. Data of mistletoes and their hosts species was collected by explorative and descriptive methods in 2013, 2017, and 2021. Recorded data by observation in the fields were species and species number of mistletoes and their hosts. Observation of the misteltoes used binoculars to ditermine the species of the mistletoes. Identification of the mistletoes species and their hosts was conducted directly in the garden and undirectly by making herbarium specimens and taking photographs. The results showed that there were five species of mistletoes infested 142 species, 82 genera and 36 families of host plants namely Dendrophthoe pentandra, Macrosolen tetragonus, Scurrula atropurpurea, Viscum articulatum, and Viscum ovalifolium. There were significantly different between the mistletoe species and their Shannon index of host plants . D. pentandra was the most generalist or the least specialist to host plants with the highest Shannon index value of 2.20±0.10, whereas S. atropurpurea was the least generalist or the most specialist with Shannon index value of 0.16±0.09.
Potential improvement in the safety and quality of traditional fermented soybean products: A narrative review Budhi Setiawan; Nugroho Eko Wirawan Budianto; Masfufatun Masfufatun
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 28 No 1 (2022): December 2022
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.28.1.20222

Abstract

Soybeans are one of the most significant crops in the world due to their nutritionally valuable. It has been converted to produce a variety of fermented soybean products. During conventional soybean fermentation, microorganisms are involved, biochemical reactions occur, and bioactive components are produced. With the rise of people's living standards, customers are paying more attention not only to the flavor and nutrition of fermented soybean meals, but also to their safety and quality. Mycotoxins, biogenic amine production, and high salt content are among the public health concerns associated with fermented soybean foods. This paper reviews the prevalent concerns about the safety and quality of fermented soybean foods, as well as potential improvements. Attempts and methods have been proposed to ensure the safety of the fermentation process and food quality. Official regulations, the employment of suitable microbes, the use of high-quality cultivars, and the administration of chemicals are all viable options for improving safety and quality. We conclude that implementing international food standards, guidelines, and codes of practice such as the Codex Alimentarius for fermented soybean products and the application of scientific novel methods (e.g., starter combination, high-pressure processing, or low-dose gamma irradiation, additive usage, low salt fermentation technique) are the potential solutions to mitigate the issues and improve the safety and quality of the products.
Life-stage dependent histological characterization of liver HIF-1α expression in the Indonesian shortfin eel (Anguilla bicolor bicolor McClelland, 1884) Nyan Hayu Widiastuti; Bambang Retnoaji
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 28 No 1 (2022): December 2022
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.28.1.20223

Abstract

The Indonesian shortfin eel (Anguilla bicolor bicolor McClelland, 1884) is catadromous fish. The developmental phase of the fish is influenced by the availability and type of feed, and dissolved oxygen levels (DO). Oxygen level fluctuations during migration from sea to fresh waters can cause hypoxia and trigger activation of the Hypoxia-inducible factor 1 alpha gene (HIF-1α). Therefore, undertanding HIF-1α expression is very important in understanding the life cycle and physiology of this organism. This study aimed to perform a histological comparison between the liver structure of eel in the elver eel phase to silver eel and compared the expression of HIF-1α in the liver. The eels were collected from Kulon Progo Regency, Segara Anakan in Cilacap, and Cangkringan in Sleman, respectively. The eel livers were prepared with standard paraffin method. The samples were stained with Hematoxylin-Eosin, Mallory Acid Fuchsin and Periodic Acid-Schiff Alcian Blue for histological observation. The HIF-1α protein expression was detected using immunohistochemistry. The results showed that the structure of liver elver, yellow, and silver eel shared similar tubular hepatocytes. Moreover, there was no significant differences in the parenchymal structure of liver of all stage of eel. Mucin levels were different in each life cycle phase. HIF-1α expression varied by eel phase, with the elver eel showing weak expression, yellow stage showing moderate expression, and silver eels displaying the highest levels of expression. We conclude that the highest expression of HIF-1α was found in the silver eel, which resides in freshwater environments and is found in freshwater while returning to the sea for reproduction.
Usnea in West Java: a potential source of bioactive secondary metabolites Miftahul Jannah; Qurrota A`yun; Nida Afifah; Eko Prasetya; Muhammad Rifqi Hariri
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 28 No 1 (2022): December 2022
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.28.1.20224

Abstract

Lichen is a symbiotic organism consisting of algae (photobionts) and fungi (mycobiont). In Indonesia, Usnea is the only genus of lichen that has been widely recognized and used by the local community for traditional medicine. It is locally known as kayu angin, rusuk angin, or janggot Kai. Furthermore, it produces lichen acid and other compounds as secondary metabolites that need investigation. Therefore, this study aimed to identify species and determine the secondary metabolites of Usnea in West Java. There were 13 species of Usnea identified, with U. rubrotincta, U. mutabilis, and U. barbata being new to West Java. The extraction of U. esperantiana has the highest number of lichen acids, with 20 different types identified. This study showed that Usnea contains lichen acid, flavonoid, and saponin compounds. The secondary metabolites are a potential source of natural products for bioprospecting.
Indole-3-butyric acid immediately induced adventitious root of Dendrodium milla nayla x Dendrobium striaenopsis planted on coco-hust and wood charcoal Tintrim Rahayu; Gatra Ervi Jayanti; Dita Agisimanto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 28 No 1 (2022): December 2022
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.28.1.20226

Abstract

Orchids are one of the most favorite cut flowers, flowering potted plants, and have developed into a highly profitable industry. The development of acclimatization method is a necessity for the high survival rate of plantlets. The aim of this study was to investigate the effect of low indole-3-butyric acid (IBA) concentration on faster root formation of Dendrobium hybrid planted on coco- husk and charcoal in a short time. Plantlets derived from the seed culture of Dendrodium milla nayla x Dendrobium striaenopsis were harvested and pre-acclimated, planted on charcoal or coco-husk, and regularly sprayed with 0.25, 0.50, 0.75, and 1.0 mg/L IBA twice a week for one month. The results showed that IBA at 0.50 mg/L provided the most appropriate concentration for immediate root induction and growth of Dendrobium hybrid planted on coco-husk. Root number (1.75) and root length (0.28 cm) showed the highest and the most important indicator of adventitious root induction
Physiological alteration and the expression of fibrogenesis-proinflammatory genes in hepatocellular carcinoma patients with chronic hepatitis C and onset metabolic syndrome: a preliminary report at Saiful Anwar General Hospital Malang Hendra Susanto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 28 No 1 (2022): December 2022
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.28.1.20225

Abstract

Hepatocellular carcinoma (HCC) is one type of cancer that is included in the fifth order of cancer that causes death in the world. The highest incidence of liver cancer is found in men. Liver function related to the metabolism of various endogenous and exogenous substances is prone to chronic inflammatory stimulation, one of which is hepatitis C virus (HCV) infection. TGF-β1, NOX4, and TNF-α are three potential genes responsible for regulating chronic inflammation and fibrogenesis. This study aims to describe the profile of potential fibrogenesis and inflammation genes that play a role in the progression of HCC due to chronic hepatitis C. This type of research is a descriptive study with a quantitative approach that was carried out using Quantitative Real-Time PCR analysis. The results of serological tests in the male gender group showed that the average fasting body glucose level was 85.22 mg/dL, total cholesterol 242 mg/dL, triglyceride 158.44 mg/dL, HDL-C 18.56 mg/dL, and LDL-C 172 mg/dL. In the female gender group, the data obtained on average levels of fasting blood glucose 82.33l mg.dL, total cholesterol 350 mg/dL, triglyceride 181 mg/dL, HDL-C 29 mg/dL, and LDL-C 190 mg/dL. All gender groups also showed an increase in AST and ALT levels that had exceeded normal limits. The expression of fibrosis-related genes, namely TGF-β1 was 5.8x higher in male patients than female patients. The NOX4 gene also showed almost the same thing, namely, its expression level was 1.2 times higher in male patients. From the expression of the proinflammatory cytokine TNF-, it is also known that in male patients, the expression level is 2x times higher than in female patients. The difference in expression levels in the two groups involved a repressive mechanism of estrogen. Increased expression levels of TGF-β1, NOX4, and TNF- genes are thought to involve the activity of the HCV core protein, which then induces several signaling pathways such as NF-κB and SMAD3. From these results, the expression levels of TGF-β1, NOX4, and TNF- as the main regulators of chronic inflammation and fibrosis can describe the progression of HCC and can be used as initial data on the profile of inflammation and fibrogenesis in HCC cases that have previously been exposed to HCV infection.
Consumer perception of insect-based foods in the community of Zhejiang University. Joko Pilianto; Xiayu Hu; Hadiatullah Hadiatullah; Tshenolo Tsheko; Asim Munawar; A.S. Abou El-Ela; Wen-Wu Zhou; Zeng-Rong Zhu
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 28 No 1 (2022): December 2022
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Insect foods are nutritious and acceptable for human consumption. The research was to determine the factors of motivation choices, satisfaction and food safety knowledge on insect foods consumption among Zhejiang University community. This study takes the form of a structured online questionnaire designed by the investigators for this convenience sample survey. Data were collected from Zhejiang University, China. A total of 873 respondents, (students, teachers and staff) ranging in age from 18 to 85 years (503 men and 370 women) completed the survey. Factor analysis was employed to identify related variables. An analysis revealed three factors; what motivates insect foods consumption by the respondents showed that taste (40.4%) is the major reason to consume insect foods followed by nutritional value (27.3%) and affordability (13.9%). Majority of the respondents were satisfied with the price of insect foods (51.7%). The survey also showed that majority of respondents did not have food safety concerns on the potential toxicity of insect (47.2%), 33% were concerned, while 19.8% did not think about the safety concerns. The study revealed that males between 18-28 years consume insect foods more than females in the same age group. Although some insect foods may be toxic, consumption is enjoyed by many of the respondents and they are not discouraged from consuming them. This study shows the current trend on insect foods consumption, bring awareness on the safety of insect foods consumption and promote insect foods consumption as some edible insects are reported to have health benefits.
Inhibitory activity of endophytic fungi against alpha-amylase isolated from raru (Cotylelobium melanoxylon) Pebrianiafrahul I Siregar; Sri Pujiyanto; Arina Tri Lunggani
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 28 No 1 (2022): December 2022
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.28.1.20227

Abstract

Diabetes mellitus is a metabolic disorder characterized by elevated blood glucose levels. Alpha-amylase inhibitors are chemicals that prevent amylase from degrading carbohydrates into monosaccharides. For instance, the Raru plant (Cotylelobium melanoxylon), which was widely used by the local people, particularly in Sumatra, was used to lower blood glucose levels. The goal of this study was to isolate an endophytic fungus from the Raru plant and test its alpha-amylase activity by optimizing treatment time, substrate concentration, and alpha-amylase inhibitor compound extraction. The activity of alpha-amylase was measured using spectrophotometry, and starch was used as the substrate. Four isolates of the endophytic fungus were isolated from Raru bark, including TR1, TR2, TR3, and TR4. Of these, the isolate TR3 had the highest alpha-amylase activity, reaching 88.71%. Alpha-amylase was optimally inhibited for 7 days with a percentage of 90.13%. At a substrate concentration of 2%, the inhibition of alpha-amylase was observed at 96.34%. While the highest extraction of alpha-amylase inhibitory compound was observed at 0.13 ppm with a percentage of 74.71% inhibition. Based on GC-MS (gas chromatography mass spectrometry) analysis, compound 24, identified as1,3-benzenedicarboxylic acid, bis (2-ethylhexyl) ester, isophthalic acid group, was produced by endophytic fungi, exerting alpha-amylase inhibitory activity. This research would provide a new source of chemical agents to combat diabetes.

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